Bioassay with waters from the Jundiaí and Tietê Rivers in the municipality of Salto- SP using the Trad-MCN method Gustavo Henrique Gonçalves, Luciana Bizeto, Claudia de Moura, Ana Beatriz Carollo Rocha-Lima

Abstract

The aim of this study was to determine the presence of potentially toxic and mutagenic agents in the pre and post industrial area in the city of Salto, in the state of São Paulo, with the objective of researching the Jundiaí and Tietê Rivers, and comparing the water quality before and after industrial region discharges. The Trad-MCN method was used, which consists on evaluating the integrity of plant chromosomes after exposure to the experimental substances. For the evaluation, approximately 100 inflorescences of Tradescantia pallida purpurea were used, which passed through chemical processes and subsequent microscopic reading of the results. The Jundiaí river presented a significant difference when compared with both controls (p <0.05). The Tietê River did not present a statistically significant difference only in the positive control (p> 0.05) and in the other control statistically significant differences were found (p <0.05). With this bioassay it was possible to attest that the water quality, which already presents toxic before the industrial region of Salto, suffers significant deterioration when passing through the industrial region, resulting in greater mutagenic activity. Recent studies done by the City Hall of Salto reclassify the Jundiaí River to class #3, former belonged to class #4 category according to Resolution CONAMA 357/2005. After the conclusion of this
bioassay, it is recommended a careful reanalysis of this reclassification by the public agencies.