Analysis of the Osmolality in Hemolinpha in Oyster (Crassostrea rhizophorae) exposed to the Atmospheric Particulated Matter Daniela Oliveira Teixeira de Sousa, Andressa dos Santos Barbosa Ortega, Marina de Souza Paço, Luis Felipe de Almeida Duarte, Iara Costa Souza, Marisa Narciso Fernandes, Camilo Dias Seabra Pereira, Helen Sadauskas-Henrique

Abstract

The iron ore industries produce steel, iron, among others, which results in atmospheric particulate matter (MPA). MPA is composed of metals/metalloids that do not have limit standards in the legislation that define the degree of water contamination. When dispersed in the atmosphere, MPA comes into contact with the aquatic environment, contaminating aquatic biota and aquatic organisms, impacting animal metabolism and behavior. The present work evaluated the interference of MPA in the regulation of osmolality in oysters (Crassostrea rhizophorae) over time. Animals were exposed to 3 MPA concentrations (0.01; 0.1 and 1 mg/L), and control (0 mg/L) for 2, 4, 7 and 30 days. At the end of the experiment, the animals were anesthetized on ice and hemolymph was collected for osmolality analysis. There were no difference in hemolymph osmolality between treatments and times. Although metals are known to cause misbalance in the osmoregulatory mechanisms, often by competing with elements such as Na+ , Cl- , Mg2+ and Ca2+, the oysters were able to maintain their solutes balance in the hemolymph