Survey of the Spectrum of Human saliva using Raman Spectroscopy Daniel Lopes Ometto, Marcos Tadeu Tavares Pacheco, Landulfo Silveira Jr.

Abstract

Raman spectroscopy is a non-destructive analytical technique that allows the identification of molecules and chemical compounds based on the interaction of light with the sample. The analysis of biofluids such as saliva is of great interest for diagnostic medicine, as they can offer a very broad map of the human metabolome. The objective of the study was to identify the main spectrum of saliva from healthy individuals using Raman spectroscopy. For this, saliva samples were collected from 20 volunteers at three different times, with seven-day intervals between each collection. The results of the study indicated the presence of the main peaks corresponding to healthy human saliva, with spectra suggestive of molecules known to be present in this fluid. These molecules are important for the functioning of the organism, being involved in processes such as protein synthesis, neurotransmission and cell signaling. Therefore, Raman spectroscopy can be a promising technique for the analysis of complex biofluids such as saliva, allowing the identification of important molecules for disease diagnosis and human health assessment.